| Intein Name |
Ssp GyrB |
| Prototype Allele |
Ssp GyrB |
| Extein Name |
DNA gyrase subunit B |
| Intein Class |
Experimental |
| Organism Name |
Synechocystis species, strain PCC6803 |
| Organism Description |
Cyanobacterium, taxon:1148 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
HNH |
| Location in Extein |
G436 |
| Insert Site Comments |
gyrB-a, Signature for DNA topoisomerase II |
| Intein Size (aa) |
435 |
| Intein N-terminal |
FIVEGDSAGG/C |
| Intein C-terminal |
HN/SAKQGRDRRF |
| Accession No. |
P77966 in NCBI/protein, sll2005 |
| Intein aa Sequence |
>gi|2501296|sp|P77966| Ssp GyrB intein (including -1 and +1 extein residues)
GCFSGDTLVALTDGRSVSFEQLVEEEKQGKQNFCYTIRHDGSIGVEKIINARKTKTNAKVIKVTLDNGES
IICTPDHKFMLRDGSYKCAMDLTLDDSLMPLHRKISTTEDSGITIDGYEMVWSPRSDSWLFTHLVADWYN
RWQGIYIAEEKQHCHHKDFNKRNNNPDNLIRLSPEKHLALHRKHISKTLHRPDVVEKCRRIHQSPEFRRK
MSARMQSPETRAILSKQAQAQWQNETYKLTMMESWRSFYDSNEDYRQQNAEQLNRAQQEYWAQAENRTAQ
AERVRQHFAQNPGLRQQYSENAVKQWNNPELLKWRQKKTKEQWTPEFREKRREALAQTYYRKTLAALKQV
EIENGYLDISAYDSYRISTKDKSLLRFDRFCERYFENDENLAREAVLNYNHRIVNIEAVSETIDVYDIEV
PHTHNFALASGVFVHNS |
| Block A |
CFSGDTLVALTDG 13 |
| Block B |
GESIICTPDHKFML 80 |
| Block C |
--------- |
| Block D |
-------- |
| Block E |
---------- |
| Block H |
HNH FAMILY ENDONUCLEASE |
| Block F |
DVYDIEV**PHTHNFA 426 |
| Block G |
SGVFVHNS 436 |
| Initially Contributed by |
Shmuel Pietrokovski |
| Contributor's Address |
Molecular Genetics Dept.
Weizmann Institute of Science
P.O. Box 26, Rehovot 76100, Israel
http://bioinfo.weizmann.ac.il/~pietro |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
Shmuel.Pietrokovski@weizmann.ac.il |
| Independently Found By |
|
| Comments |
This intein contains a motif typical to HNH type homing endonucleases instead of the more common DOD type homing endonucleases (Pietrokovski). |
| Date Submitted |
05/16/1997 |
| References |
Kaneko 1995
Kaneko 1996
Kaneko 1996B
Kaneko 1997
Dalgaard 1997
Pietrokovski 1998
Gorbalenya 1998
Appleby 2009 |
