| Intein Name |
MP-Omega DnaB |
| Prototype Allele |
|
| Extein Name |
DnaB helicase |
| Intein Class |
Theoretical |
| Organism Name |
Mycobacteriophage Omega |
| Organism Description |
Bacteriophage, taxon:205879 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K106 |
| Insert Site Comments |
dnaB-e |
| Intein Size (aa) |
340 |
| Intein N-terminal |
LIIHSAHEFK/A |
| Intein C-terminal |
HN/TAVNGMERLE |
| Accession No. |
AAN12655.1 in NCBI/protein,gp11 |
| Intein aa Sequence |
>gi|29425473|gb|AAN12655.1|[29425473] MP-Omega DnaB intein (including -1 and +1 extein residues)
KALAIDTPILSERGWTTMGDLVDGDRVYGPDGQLTNVIAHPIRYERPCYRLTFDDGQTIV
ADEDHLWTVYDAVKREHRTLTVRELVDGGVFTTRRNAGRADSNIYRYRVPVTEPLAGVEA
DLPVDPYLLGYWLGDGDTNAGRFTVGEEDLEAFKLTLESLGYEYSDSVDPRTGAHTICAY
GFIQGLREAGVVGNKHIPETYLTASMEQRRALLAGIMDSDGGVTGHQISVTMKNEALMRQ
VLMLARSLGYKSFFTSHLSMLNGEHKARVYRVKFANRQELNPFRLPRKAAKVLPPLGRVT
RAQYNAIVSIEPVESVPTRCITVDNDSRLYVVGHGFVPTHNT |
| Block A |
ALAIDTPILSERG 13 |
| Block B |
GQTIVADEDHLWTV 68 |
| Block C |
LLGYWLGDG 135 |
| Block D |
NKHIPETY 200 |
| Block E |
LLAGIMDSDG 220 |
| Block H |
MKNEALMRQVLMLARSLGY 259 |
| Block F |
PTRCITV*DNDSRLYV 330 |
| Block G |
GFVPTHNT 341 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
New England Biolabs, Inc. |
| Contributor's Phone No. |
1-978-927-5054 |
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
perler@neb.com |
| Independently Found By |
|
| Comments |
Has an Ala instead of the conserved intein N-terminal Ser/Cys.
Note that the Momega and Mcjw1 DnaB inteins are very similar, but inserted into different K/T sites in the phage DnaB extein. |
| Date Submitted |
04/28/2003 |
| References |
Pedulla 2003 |
