New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Cga PRP8 Intein
Intein Name Cga PRP8
Prototype Allele Cne-AD PRP8
Extein Name PRP8, pre-mRNA splicing factor
Intein Class Theoretical
Organism Name Cryptococcus gattii (aka Cryptococcus bacillisporus)
Organism Description Yeast, human pathogen
Domain of Life Eucarya
Endonuclease Activity
Endo Motif None
Location in Extein A?
Insert Site Comments PRP8-a, same as A1578 in Sce PRP8
Intein Size (aa) 170
Intein N-terminal TWEGLFWEKA/C
Intein C-terminal HN/SGFEESMKNK
Accession No. AAR00331.1 in NCBI/protein,
Intein aa Sequence >gi|37695593|gb|AAR00331.1|CbaPRP8_WM02.98| Cga PRP8 intein (including -1 and +1 extein residues)
ACLQNGTRLLRADGSEILVEDVQEGDQLLGPDGTSRTASKIVRGEERLYRIKADELEDLVCTHNHILSL
YKKRSGSEQDPSPSTDLSSTDSYERVDVTVDDFVRLPQQEQQKYRLFRSTGFKRADQPSTSSLATLLHI
MSIQLEEKPTKWSGFVVVKDSLYLRHDYLVLHNS
Block A CLQNGTRLLRADG 13
Block B LEDLVCTHNHILSL 68
Block C None
Block D None
Block E None
Block H None
Block F PTKWSGFVVDKDSLYL 161
Block G DYLVLHNS 171
Initially Contributed by Margi Butler
Contributor's Address Department of Biochemistry
University of Otago
PO Box 56
Dunedin
New Zealand/Aotearoa
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address margi.butler@stonebow.otago.ac.nz
Independently Found By
Comments The organism is also called Cryptococcus neoformans var gattii and Cryptococcus bacillisporus.
This gene also contains an intron. The PRP8 inteins from numerous Cga strains form 4 clades based on DNA sequence.
Date Submitted 04/27/2003
References Butler 2005

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home