| Intein Name |
Tfu Pol-1 |
| Prototype Allele |
Pko Pol-1 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Thermococcus fumicolans |
| Organism Description |
Thermophilem, taxon:46540 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
PI-TfuI |
| Endo Motif |
DOD |
| Location in Extein |
R406 |
| Insert Site Comments |
pol-a, Pol Motif A |
| Intein Size (aa) |
360 |
| Intein N-terminal |
WENIAYLDFR/C |
| Intein C-terminal |
HN/SLYPSIIISH |
| Accession No. |
P74918 in NCBI/protein |
| Intein aa Sequence |
>gi|3913528|sp|P74918| Tfu Pol-1 intein (including -1 and +1 extein residues)
RCHPADTKVIVKGKGVVNISEVREGDYVLGIDGWQKVQRVWEYDYEGELVNINGLKCTPNHKLPVVRRTE
RQTAIRDSLAKSFLTKKVKGKLITTPLFEKIGKIEREDVPEEEILKGELAGIILAEGTLLRKDVEYFDSS
RGKKRVSHQYRVEITVGAQEEDFQRRIVYIFERLFGVTPSVYRKKNTNAITFKVAKKEVYLRVREIMDGI
ENLHAPSVLRGFFEGDGSVNKVRKTVVVNQGTNNEWKIEVVSKLLNKLGIPHRRYTYDYTEREKTMTTHI
LEIAGRDGLILFQTIVGFISTEKNMALEEAIRNREVNRLENNAFYTLADFTAKTEYYKGKVYDLTLEGTP
YYFANGILTHNS |
| Block A |
CHPADTKVIVKGK 13 |
| Block B |
INGLKCTPNHKLPV 64 |
| Block C |
LAGIILAEG 126 |
| Block D |
VREIMDGI 209 |
| Block E |
VLRGFFEGDG 224 |
| Block H |
TNNEWKIEVVSKLLNKLGI 259 |
| Block F |
KVYDLTL**EGTPYYF 352 |
| Block G |
NGILTHNS 361 |
| Initially Contributed by |
Joel Querellou |
| Contributor's Address |
IFREMER, DRV/VP/BMH
BP 70, 29282 PLOUZANE
FRANCE |
| Contributor's Phone No. |
33-2 98 22 46 11 |
| Contributor's FAX No. |
33-2 98 22 45 47 |
| Contributor's Email address |
jquerell@ifremer.fr |
| Independently Found By |
|
| Comments |
Direct submission to GenBank. |
| Date Submitted |
02/14/1997 |
| References |
Saves 2000
Cambon-Bonavita 2000
Saves 2000C
Thion 2002 |
