| Intein Name |
Bde-JEL197 RPB2 |
| Prototype Allele |
Bde-JEL197 RPB2 |
| Extein Name |
DNA-directed RNA polymerase II, second largest subunit |
| Intein Class |
Theoretical |
| Organism Name |
Batrachochytrium dendrobatidis JEL197 |
| Organism Description |
Chytrid fungus, isolate="AFTOL-ID 21", taxon:109871 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
V120? |
| Insert Site Comments |
RPB2-c, domain 3, V522 in Sce RPB2 |
| Intein Size (aa) |
488 |
| Intein N-terminal |
QLHNTHWGMV/C |
| Intein C-terminal |
HN/CPAETPEGQA |
| Accession No. |
ABC17934 in NCBI/protein |
| Intein aa Sequence |
>gi|83415453|gb|ABC17934.1|[83415453] Bde-JEL197 RPB2 intein (including -1 and +1 extein residues)
VCLSGDTEVVLGNGMDVVLLTDLVDGACVSTIEPRTGVVSPSLIDHYFAKDATRVLKITLDDGRVIKADPEHPLFAAKVD
ETTRGLTGQWLRVEELTVGHHALLVSPQQAYCTSEQWTLTLSEKEVCTASTAQLAKQLSEIGLVGVPIPLSKVKSAARLF
GLVLSAGDFSGKLYVEKDEDVTAVNSDLTALGFSPAHWVRYQDDQQKWFKCDDSNGKAICITLVPTANALLQTLGAHDAE
KQHRYCLLPKWLLDAPTSIKREFLGALFGGNGAHITISCNEGKWEPTMSALTQHTDADHLESTVVYLKQVATLLGMLNIC
SSVSSEKHITAEKTGYAVHLHVDNTAENLVRFYEQVGYRYCFNKTSQSSAPVQWIKGSLFFIEQHRSKCQHAFELLHTGL
GTKAAVSAEINMPHHNPSHMLNVKEIPTAPADEYITWIEFKDRYVHKESPRFVWVTIHSIEEAPAERLYDFNTVSQNHSF
FANSIVSHNC |
| Block A |
CLSGDTEVVLGNG 13 |
| Block B |
GRVIKADPEHPLFA 75 |
| Block C |
LFGLVLSAG 166 |
| Block D |
YCLLPKWL 251 |
| Block E |
FLGALFGGNG 271 |
| Block H |
ESTVVYLKQVATLLGMLNI 318 |
| Block F |
RLYDFNTV*SQNHSFF 480 |
| Block G |
NSIVSHNC 489 |
| Initially Contributed by |
Tim Goodwin |
| Contributor's Address |
Department of Biochemistry
University of Otago
710 Cumberland Street
Dunedin,New Zealand |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
timg@sanger.otago.ac.nz |
| Independently Found By |
|
| Comments |
The Bde-JEL423 RPC2 and Bde-JEL197 RPB2 local insertion site sequences are very similar, as are the intein sequences. However, the remainder of the exteins are not similar. Therefore, it looks like intein alleles have been recently transferred to non-allelic exteins. Insertion site listed for partial extein sequence. Insertion site at equivalent position of V522 in Sce RPB2 CAA99357. |
| Date Submitted |
04/09/2006 |
| References |
|
