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Pho Lon Intein
Intein Name Pho Lon
Prototype Allele Pho Lon
Extein Name ATP-dependent protease LA
Intein Class Theoretical
Organism Name Pyrococcus horikoshii OT3
Organism Description Thermophile, taxon:53953
Domain of Life Archaea
Endonuclease Activity
Endo Motif DOD
Location in Extein Q210
Insert Site Comments lon-a
Intein Size (aa) 474
Intein N-terminal LGDVRHDPFQ/C
Intein C-terminal KN/SGGLGTPAHL
Accession No. BAA29538.1 in NCBI/protein, PH0452
Intein aa Sequence >gi|3256855|dbj|BAA29538.1| Pho Lon intein (including -1 and +1 extein residues)
QCFSGEEVIIVEKGKDRKVVKLREFVEDALKEPSGEGMDGDIKVTYKDLRGEDVRILTKDGFVKLLYVNK
REGKQKLRKIVNLDKDYWLAVTPDHKVFTSEGLKEAGEITEKDEIIRVPLVILDGPKIASTYGEDGKFDD
YIRWKKYYEKTGNGYKRAAKELNIKESTLRWWTQGAKPNSLKMIEELEKLNLLPLTSEDSRLEKVAIILG
ALFSDGNIDRNFNTLSFISSERKAIERFVETLKELFGEFNYEIRDNHESLGKSILFRTWDRRIIRFFVAL
GAPVGNKTKVKLELPWWIKLKPSLFLAFMDGLYSGDGSVPRFARYEEGIKFNGTFEIAQLTDDVEKKLPF
FEEIAWYLSFFGIKAKVRVDKTGDKYKVRLIFSQSIDNVLNFLEFIPISLSPAKREKFLREVESYLAAVP
ESSLAGRIEELREHFNRIKKGERRSFIETWEVVNVTYNVTTETGNLLANGLFVKNS
Block A CFSGEEVIIVEKG 13
Block B DYWLAVTPDHKVFT 98
Block C ILGALFSDG 215
Block D KLELPWWI 297
Block E FMDGLYSGDG 316
Block H EKKLPFFEEIAWYLSFFGI 362
Block F VTYNVTT***ETGNLL 466
Block G NGLFVKNS 475
Initially Contributed by Fran Perler
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Independently Found By
Comments Intein insertion site numbered using Met1 as listed in above Acc. No.
Has a Lys instead of His in Block G.
Motifs by Pietrokovski and Perler
Date Submitted 07/20/1998
References Kawarabayasi 1998
Kawarabayasi 1998B
Kawarabayasi 2001

Last database update: 11/05/10

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