New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Tth-HB8 DnaE-2 Intein
Intein Name Tth-HB8 DnaE-2
Prototype Allele Tth-HB8 DnaE-2
Extein Name DnaE pol subunit, DNA polymerase III alpha subunit
Intein Class Theoretical
Organism Name Thermus thermophilus HB8
Organism Description thermophile, taxon:300852
Domain of Life Eubacteria
Endonuclease Activity
Endo Motif DOD
Location in Extein K1238
Insert Site Comments dnaE-c
Intein Size (aa) 423
Intein N-terminal EAFANYGFNK/C
Intein C-terminal HN/SHAAAYSLLS
Accession No. YP_143446 in NCBI/protein, TTHA0180
Intein aa Sequence >gi|55980149|ref|YP_143446.1|[55980149] Tth-HB8 DnaE-2 intein (including -1 and +1 extein residues)
KCLPARARVVDWCTGRVVRVGEIVRGEAKGVWVVSLDEARLRLVPRPVVAAFPSGKAQVYALRTATGRVLEATANHPVYT
PEGWRPLGTLAPGDYVALPRHLSYRPSLHLEGHELDLLGFALAEGHLRHPSGVYLYTSSEEELAAMEEALRAFPNTRIRV
VWRRGVAHVYVGRVDRRQEAGAVAFLRRMGLLGLDAKTKRLPEAVFGLPPEEVARFLGRLWTGDGGVDPKGRLIHYATAS
KELAWGVQHLLLRLGLQSRLVEKRFSGGYKGYAVYLLGGLEAARRFAETVGPYLVGKRRQDLEALLASWEKAGRSTRDVL
PLAFLEEVRAAVAEVAQGQVADLLREAGLAEGLLCLGRGRRGLSRATVGRLAALTGSLALLRLAEAEVYWDRVEAVEPLG
EEEVFDLTVEGTHTFVAEDVIVHNS
Block A CLPARARVVDWCT 13
Block B GRVLEATANHPVYT 79
Block C
Block D
Block E
Block H
Block F EVFDLTV**EGTHTFV 415
Block G EDVIVHNS 424
Initially Contributed by Fran Perler
Contributor's Address
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address
Independently Found By
Comments Homolog of the catalytic alpha subunit of E. coli DNA polymerase III.
Date Submitted 02/22/2005
References

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home