| Intein Name |
Pfu TopA |
| Prototype Allele |
Mja r-Gyr |
| Extein Name |
DNA topoisomerase I |
| Intein Class |
Theoretical |
| Organism Name |
Pyrococcus furiosus |
| Organism Description |
Thermophile, taxon:186497, DSM3638 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
F314 |
| Insert Site Comments |
top-a, 2 aa before the catalytic Y residue |
| Intein Size (aa) |
373 |
| Intein N-terminal |
IAQSLYEKGF/C |
| Intein C-terminal |
HN/CSYPRTESQK |
| Accession No. |
AAC26106.1 in NCBI/protein, PF0494 |
| Intein aa Sequence |
>gi|3309559|gb|AAC26106.1| Pfu TopA intein (including -1 and +1 extein residues)
FCLHPDTLILTSQGVRKIKELSREGEVFALDFNLKLSKAKYRLLERDADEQMYKVTLLDGTELYLTADHP
VLVYREGNLAFVPADKLRETDHVVLVLNKSARDNYGFLDLLLEITDSQEDYAILENGETLSLHSLKMLVE
RGEIKDIAVVGFSHNNFGKVMLRDELWYLIGYLAGKGGEIKGNGVVISSRTKEIVGLTKSLNIDLIETEE
GIVLSNKSFVRLLHLIHYTPRVPEVYGIINNTEWLKAFLAGYYDATLLEGLTLEALYKIKVYLQLLGIRA
KIEDNKLKVHLEDLQRFRELLGKFSRRKLYVETSQVPVFTDFDERSYDFPRILGGDIYIIGIKSIEKFHY
KGKVYDLVVENYHNFIANGIAVHNC |
| Block A |
CLHPDTLILTSQG 13 |
| Block B |
GTELYLTADHPVLV 72 |
| Block C |
LIGYLAGKG 176 |
| Block D |
-------- |
| Block E |
FLAGYYDATL 256 |
| Block H |
GLTLEALYKIKVYLQLLGI 277 |
| Block F |
KVYDLVV**ENYHNFI 365 |
| Block G |
NGIAVHNC 374 |
| Initially Contributed by |
Paul Xiang-Qin Liu |
| Contributor's Address |
Biochemistry Department
Dalhousie University
Halifax, NS, Canada B3H 4H7 |
| Contributor's Phone No. |
902-494-1208 |
| Contributor's FAX No. |
902-494-1355 |
| Contributor's Email address |
pxqliu@dal.ca |
| Independently Found By |
|
| Comments |
This intein and the Mja r-Gyr intein have the same insertion site in their exteins and have a low level of sequence identity; the exteins have related domains, but are functionally distinct (Liu). |
| Date Submitted |
11/25/1997 |
| References |
Chute 1998
Maeder 1999 |
