| Intein Name |
Kex-CBS379 VMA |
| Prototype Allele |
Sce VMA |
| Extein Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein Class |
Theoretical |
| Organism Name |
Kazachstania exigua, formerly Saccharomyces exiguus, strain CBS379 |
| Organism Description |
Yeast, taxon:34358 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G18? |
| Insert Site Comments |
VMA-a |
| Intein Size (aa) |
502 |
| Intein N-terminal |
SNSDAIIYVG/C |
| Intein C-terminal |
HN/CGERGNEMAE |
| Accession No. |
CAC86353.1 or BAC66653 in NCBI/protein,gene="VMA1-2 |
| Intein aa Sequence |
>gi|27528478|emb|CAC86353.1|[27528478] Kex-CBS379 VMA intein (including -1 and +1 extein residues)
GCFAKGTQVMMADGTDKSIEDIQLGENVMGKDGTPRTVISLPRGKETMYEVCHSSIKGTT
NETLMNYVCSGNHKIVMQTPQQIGITEHAIDGKTYTSVSYFALRDSQDGYPIVKKLTKSF
EHQQNGGKEQTLITVNNFVASLKSEPINWDVEAKHYESLGHYVKKCSYQLINPVFHQSGK
LAQEIASLNYSKDKAPLLAWLIGFWVGNGAMRHSQFVIDSQDIELVNRIQEIATLLNLSA
TTACYYSGSVDDQDKLAKLNTATGEGFEDDLQSNPTAKELADMDILSESKTATLPTAFGV
EKIDELIISLGEGRGNGNVFAQVIGSFGIDLADKDTLVSKIAHELAGDAFEVREQFVAGL
VDATGYVRKDVYDHASEATVSIESKSTVLGLVKIARSLGIKIVVTSNDYQDPEDHDHGHD
HGSCGHDEVATGYTAVMTGDALTNSMRFCAIGRNRVTAKQFMRQAIPFYFSLEKKSEDDY
YGITVPDNTDKQYLLSSMALVHNC |
| Block A |
CFAKGTQVMMADG 13 |
| Block B |
LMNYVCSGNHKIVM 76 |
| Block C |
LIGFWVGNG 208 |
| Block D |
VSKIAHEL 344 |
| Block E |
FVAGLVDATG 364 |
| Block H |
IESKSTVLGLVKIARSLGI 399 |
| Block F |
YGITVP**DNTDKQYL 493 |
| Block G |
SMALVHNC 503 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
Koufopanou V., Biology, Imperial College at Silwood Park, Ascot, Berks, SL5 7PY, UNITED KINGDOM |
| Comments |
Okuda 2003 reports 2 distinct VMA intein alleles in strains IFO0316 and IFO1128. |
| Date Submitted |
04/28/2003 |
| References |
Koufopanou 2002
Okuda 2003 |
|
