| Intein Name |
Psp-GBD Pol |
| Prototype Allele |
Tli Pol-1 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Pyrococcus species GB-D |
| Organism Description |
Thermophile |
| Domain of Life |
Archaea |
| Endonuclease Activity |
PI-PspI |
| Endo Motif |
DOD |
| Location in Extein |
N492 |
| Insert Site Comments |
pol-b, Pol Motif B |
| Intein Size (aa) |
537 |
| Intein N-terminal |
RQRAIKILAN/S |
| Intein C-terminal |
HN/SYYGYYGYAK |
| Accession No. |
AAA67132.1 in NCBI/protein |
| Intein aa Sequence |
>gi|436494|gb|AAA67132.1|[436494] Psp-GBD Pol intein (including -1 and +1 extein residues)
NSILPEEWVPLIKNGKVKIFRIGDFVDGLMKANQGKVKKTGDTEVLEVAGIHAFSFDRKSKKARVMAVKA
VIRHRYSGNVYRIVLNSGRKITITEGHSLFVYRNGDLVEATGEDVKIGDLLAVPRSVNLPEKRERLNIVE
LLLNLSPEETEDIILTIPVKGRKNFFKGMLRTLRWIFGEEKRVRTASRYLRHLENLGYIRLRKIGYDIID
KEGLEKYRTLYEKLVDVVRYNGNKREYLVEFNAVRDVISLMPEEELKEWRIGTRNGFRMGTFVDIDEDFA
KLLGYYVSEGSARKWKNQTGGWSYTVRLYNENDEVLDDMEHLAKKFFGKVKRGKNYVEIPKKMAYIIFES
LCGTLAENKRVPEVIFTSSKGVRWAFLEGYFIGDGDVHPSKRVRLSTKSELLVNGLVLLLNSLGVSAIKL
GYDSGVYRVYVNEELKFTEYRKKKNVYHSHIVPKDILKETFGKVFQKNISYKKFRELVENGKLDREKAKR
IEWLLNGDIVLDRVVEIKREYYDGYVYDLSVDEDENFLAGFGFLYAHNS |
| Block A |
SILPEEWVPLIKN 13 |
| Block B |
GRKITITEGHSLFV 100 |
| Block C |
LLGYYVSEG 289 |
| Block D |
NKRVPEVI 364 |
| Block E |
FLEGYFIGDG 384 |
| Block H |
TKSELLVNGLVLLLNSLGV 414 |
| Block F |
YVYDLSV**DEDENFL 527 |
| Block G |
GFLYAHNS 538 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
New England Biolabs |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
perler@neb.com |
| Independently Found By |
|
| Comments |
Formally called Psp Pol-1 |
| Date Submitted |
|
| References |
Xu 1993
Xu 1994
Shao 1995
Xu 1996
Shao 1996
Southworth 1998 |
