| Intein Name |
Tko Pol-2 (Pko Pol-2) |
| Prototype Allele |
Tli Pol-1 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Pyrococcus/Thermococcus kodakaraensis KOD1 |
| Organism Description |
Thermophile, taxon:69014 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
PI-PkoII |
| Endo Motif |
DOD |
| Location in Extein |
N851 |
| Insert Site Comments |
pol-b, Pol Motif B |
| Intein Size (aa) |
536 |
| Intein N-terminal |
RQRAIKILAN/S |
| Intein C-terminal |
HN/SYYGYYGYAR |
| Accession No. |
S71551 in NCBI/protein |
| Intein aa Sequence |
>gi|2129415|pir||S71551|P77933 Tko Pol-2 (Psp KOD Pol-2) intein (including -1 and +1 extein residues)
NSILPEEWLPVLEEGEVHFVRIGELIDRMMEENAGKVKREGETEVLEVSGLEVPSFNRRTNKAELKRVKA
LIRHDYSGKVYTIRLKSGRRIKITSGHSLFSVRNGELVEVTGDELKPGDLVAVPRRLELPERNHVLNLVE
LLLGTPEEETLDIVMTIPVKGKKNFFKGMLRTLRWIFGEEKRPRTARRYLRHLEDLGYVRLKKIGYEVLD
WDSLKNYRRLYEALVENVRYNGNKREYLVEFNSIRDAVGIMPLKELKEWKIGTLNGFRMRKLIEVDESLA
KLLGYYVSEGYARKQRNPKNGWSYSVKLYNEDPEVLDDMERLASRFFGKVRRGRNYVEIPKKIGYLLFEN
MCGVLAENKRIPEFVFTSPKGVRLAFLEGYSSAMATSTEQETQALNEKRALANQLVLLLNSVGVSAVKLG
HDSGVYRVYINEELPFVKLDKKKNAYYSHVIPKEVLSEVFGKVFQKNVSPQTFRKMVEDGRLDPEKAQRL
SWLIEGDVVLDRVESVDVEDYDGYVYDLSVEDNENFLVGFGLVYAHNS |
| Block A |
SILPEEWLPVLEE 13 |
| Block B |
GRRIKITSGHSLFS 100 |
| Block C |
LLGYYVSEG 289 |
| Block D |
NKRIPEFV 364 |
| Block E |
FLEGYFIGDG 384 |
| Block H |
NEKRALANQLVLLLNSVGV 413 |
| Block F |
YVYDLSV**EDNENFL 526 |
| Block G |
GLVYAHNS 537 |
| Initially Contributed by |
F.Perler for Tadayuki Imanaka |
| Contributor's Address |
Osaka University
Dept. of Biotechnology
2-1 Yamadaoka, Suita, Osaka 565
Japan |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
|
| Independently Found By |
|
| Comments |
Formally called Pko Pol-2, Psp Pol-3 or Psp KOD Pol-2
This organism is now classified as Thermococcus kodakaraensis. |
| Date Submitted |
07/08/1996 |
| References |
Takagi 1997
Nishioka 1998
Hashimoto 2000
Fukui 2005 |
