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Tko Pol-2 (Pko Pol-2) Intein
Intein Name Tko Pol-2 (Pko Pol-2)
Prototype Allele Tli Pol-1
Extein Name DNA polymerase (alpha or family B)
Intein Class Experimental
Organism Name Pyrococcus/Thermococcus kodakaraensis KOD1
Organism Description Thermophile, taxon:69014
Domain of Life Archaea
Endonuclease Activity PI-PkoII
Endo Motif DOD
Location in Extein N851
Insert Site Comments pol-b, Pol Motif B
Intein Size (aa) 536
Intein N-terminal RQRAIKILAN/S
Intein C-terminal HN/SYYGYYGYAR
Accession No. S71551 in NCBI/protein
Intein aa Sequence >gi|2129415|pir||S71551|P77933 Tko Pol-2 (Psp KOD Pol-2) intein (including -1 and +1 extein residues)
NSILPEEWLPVLEEGEVHFVRIGELIDRMMEENAGKVKREGETEVLEVSGLEVPSFNRRTNKAELKRVKA
LIRHDYSGKVYTIRLKSGRRIKITSGHSLFSVRNGELVEVTGDELKPGDLVAVPRRLELPERNHVLNLVE
LLLGTPEEETLDIVMTIPVKGKKNFFKGMLRTLRWIFGEEKRPRTARRYLRHLEDLGYVRLKKIGYEVLD
WDSLKNYRRLYEALVENVRYNGNKREYLVEFNSIRDAVGIMPLKELKEWKIGTLNGFRMRKLIEVDESLA
KLLGYYVSEGYARKQRNPKNGWSYSVKLYNEDPEVLDDMERLASRFFGKVRRGRNYVEIPKKIGYLLFEN
MCGVLAENKRIPEFVFTSPKGVRLAFLEGYSSAMATSTEQETQALNEKRALANQLVLLLNSVGVSAVKLG
HDSGVYRVYINEELPFVKLDKKKNAYYSHVIPKEVLSEVFGKVFQKNVSPQTFRKMVEDGRLDPEKAQRL
SWLIEGDVVLDRVESVDVEDYDGYVYDLSVEDNENFLVGFGLVYAHNS
Block A SILPEEWLPVLEE 13
Block B GRRIKITSGHSLFS 100
Block C LLGYYVSEG 289
Block D NKRIPEFV 364
Block E FLEGYFIGDG 384
Block H NEKRALANQLVLLLNSVGV 413
Block F YVYDLSV**EDNENFL 526
Block G GLVYAHNS 537
Initially Contributed by F.Perler for Tadayuki Imanaka
Contributor's Address Osaka University
Dept. of Biotechnology
2-1 Yamadaoka, Suita, Osaka 565
Japan
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address
Independently Found By
Comments Formally called Pko Pol-2, Psp Pol-3 or Psp KOD Pol-2
This organism is now classified as Thermococcus kodakaraensis.
Date Submitted 07/08/1996
References Takagi 1997
Nishioka 1998
Hashimoto 2000
Fukui 2005

Last database update: 11/05/10

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