| Intein Name |
Pfu RIR1-2 |
| Prototype Allele |
Pfu RIR1-2 |
| Extein Name |
Ribonucleoside- diphosphate reductase, alpha subunit |
| Intein Class |
Experimental |
| Organism Name |
Pyrococcus furiosus |
| Organism Description |
Thermophile, taxon:186497, DSM3638 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
PI-PfuII |
| Endo Motif |
DOD |
| Location in Extein |
P914 |
| Insert Site Comments |
RIR1-b |
| Intein Size (aa) |
382 |
| Intein N-terminal |
KGGPIRATNP/C |
| Intein C-terminal |
HN/CGEEPLYEYE |
| Accession No. |
AAB36947.1 in NCBI/protein, PF0440 |
| Intein aa Sequence |
>gi|1688292|gb|AAB36947.1| Pfu RIR1-2 intein (including -1 and +1 extein residues)
PCVVGDTRILTPEGYLKAEEIFSLAKERGKKEAVAVEGIAEEGEPYAYSVEILLPGEEKVEYETVHGKVL
AVADPVAVPAYVWKVGRKKVARVKTKEGYEITATLDHKLMTPEGWKEVGKLKEGDKILLPRFEVEEEFGS
ESIGEDLAFVLGWFIGDGYLNVNDKRAWFYFNAEKEEEIAVRIRDILVKHFGIKAELHRYGNQIKLGVRG
EAYRWLENIVKNNEKRIPEIVYRLKPREIAAFLRGLFSADGYVDKDMAIRLTSKSRELLREVQDLLLLFG
ILSKIYEKPYESEFHYTTKNGEERIYRSKGYYELVITNYSRKLFAEKIGLEGYKMEKLSLKKTKVDQPIV
TVESVEVLGEEIVYDFTVPNYHMYISNGFMSHNC |
| Block A |
CVVGDTRILTPEG 13 |
| Block B |
EEKVEYETVHGRVL 69 |
| Block C |
VLGWFIGDG 157 |
| Block D |
EKRIPEIV 230 |
| Block E |
FLRGLFSADG 250 |
| Block H |
SKSRELLREVQDLLLLFGI 280 |
| Block F |
IVYDFTV**PNYHMYI 374 |
| Block G |
NGFMSHNC 383 |
| Initially Contributed by |
Frank Robb |
| Contributor's Address |
Center of Marine Biotechnology
University of Maryland
701 E. Pratt St
Baltimore, MD 21202, USA |
| Contributor's Phone No. |
1-410 234 8803 |
| Contributor's FAX No. |
1-410 234 8896 |
| Contributor's Email address |
robb@umbi.umd.edu |
| Independently Found By |
|
| Comments |
This intein is inserted in the same position in the RIR1 extein as Mth RIR1, but the 2 inteins are not very similar (Pietrokovski). Endonuclease information from Y. Ishino. |
| Date Submitted |
|
| References |
Riera 1997
Maeder 1999
Komori 1999
Komori 1999B
Ottomo 1999B |
