New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Pfu RIR1-2 Intein
Intein Name Pfu RIR1-2
Prototype Allele Pfu RIR1-2
Extein Name Ribonucleoside- diphosphate reductase, alpha subunit
Intein Class Experimental
Organism Name Pyrococcus furiosus
Organism Description Thermophile, taxon:186497, DSM3638
Domain of Life Archaea
Endonuclease Activity PI-PfuII
Endo Motif DOD
Location in Extein P914
Insert Site Comments RIR1-b
Intein Size (aa) 382
Intein N-terminal KGGPIRATNP/C
Intein C-terminal HN/CGEEPLYEYE
Accession No. AAB36947.1 in NCBI/protein, PF0440
Intein aa Sequence >gi|1688292|gb|AAB36947.1| Pfu RIR1-2 intein (including -1 and +1 extein residues)
PCVVGDTRILTPEGYLKAEEIFSLAKERGKKEAVAVEGIAEEGEPYAYSVEILLPGEEKVEYETVHGKVL
AVADPVAVPAYVWKVGRKKVARVKTKEGYEITATLDHKLMTPEGWKEVGKLKEGDKILLPRFEVEEEFGS
ESIGEDLAFVLGWFIGDGYLNVNDKRAWFYFNAEKEEEIAVRIRDILVKHFGIKAELHRYGNQIKLGVRG
EAYRWLENIVKNNEKRIPEIVYRLKPREIAAFLRGLFSADGYVDKDMAIRLTSKSRELLREVQDLLLLFG
ILSKIYEKPYESEFHYTTKNGEERIYRSKGYYELVITNYSRKLFAEKIGLEGYKMEKLSLKKTKVDQPIV
TVESVEVLGEEIVYDFTVPNYHMYISNGFMSHNC
Block A CVVGDTRILTPEG 13
Block B EEKVEYETVHGRVL 69
Block C VLGWFIGDG 157
Block D EKRIPEIV 230
Block E FLRGLFSADG 250
Block H SKSRELLREVQDLLLLFGI 280
Block F IVYDFTV**PNYHMYI 374
Block G NGFMSHNC 383
Initially Contributed by Frank Robb
Contributor's Address Center of Marine Biotechnology
University of Maryland
701 E. Pratt St
Baltimore, MD 21202, USA
Contributor's Phone No. 1-410 234 8803
Contributor's FAX No. 1-410 234 8896
Contributor's Email address robb@umbi.umd.edu
Independently Found By
Comments This intein is inserted in the same position in the RIR1 extein as Mth RIR1, but the 2 inteins are not very similar (Pietrokovski). Endonuclease information from Y. Ishino.
Date Submitted
References Riera 1997
Maeder 1999
Komori 1999
Komori 1999B
Ottomo 1999B

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home