| Intein Name |
Sce-DH1-1A VMA |
| Prototype Allele |
Sce VMA |
| Extein Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein Class |
Theoretical |
| Organism Name |
Saccharomyces cerevisiae strain DH1-1A |
| Organism Description |
Yeast,taxon:173900,also in Sce strains OUT7900,OUT7903,OUT7112 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G28? |
| Insert Site Comments |
VMA-a |
| Intein Size (aa) |
454 |
| Intein N-terminal |
SNSDAIIYVGlC |
| Intein C-terminal |
HN/CGERGNEMAE |
| Accession No. |
AAL18609 in NCBI/protein |
| Intein aa Sequence |
>gi|16417188|gb|AAL18609.1|[16417188] Sce-DH1-1A VMA intein (including -1 and +1 extein residues)
GCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGSETMYSVVQKSQHRAHKSDSSREMPELLKFTCNATH
ELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPVSEGPERANELVESYRKASNKAYFEWTI
EARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERV
TEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGVRNNLNTENPLWDAIIGLGFLKDGVKNIPSFLSTDNIGTRETFL
AGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVASVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCA
GSKKFRPAPVATFVRECQGFYFELQELKENDYYGITLSDDSDHQFLLANQVVVHNC |
| Block A |
CFAKGTNVLMADG 13 |
| Block B |
LLKFTCNATHELVV 83 |
| Block C |
LLGLWIGDG 219 |
| Block D |
VKNIPSFL 307 |
| Block E |
FLAGLIDSDG 327 |
| Block H |
TIHTSVRDGLVSLARSLGL 359 |
| Block F |
YGITLS**DDSDHQFL 445 |
| Block G |
NQVVVHNC 455 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
Same intein aa sequence in Sce strain OUT7900 (BAC66644.1), Sce strain OUT7903 (BAC66645.1), and Sce strain OUT7112 (BAC66643.1) |
| Date Submitted |
02/24/2005 |
| References |
Gimble 2001 |
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