| Intein Name |
Pfu RIR1-1 |
| Prototype Allele |
Pfu RIR1-1 |
| Extein Name |
Ribonucleoside- diphosphate reductase, alpha subunit |
| Intein Class |
Experimental |
| Organism Name |
Pyrococcus furiosus |
| Organism Description |
Thermophile, taxon:186497, DSM3638 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
PI-PfuI |
| Endo Motif |
DOD |
| Location in Extein |
G301 |
| Insert Site Comments |
RIR1-a |
| Intein Size (aa) |
454 |
| Intein N-terminal |
VAMIQKMGGG/C |
| Intein C-terminal |
HN/TGLNFSKLRP |
| Accession No. |
AAB36947.1 in NCBI/protein, PF0440 |
| Intein aa Sequence |
>gi|1688292|gb|AAB36947.1| Pfu RIR1-1 intein (including -1 and +1 extein residues)
GCIDGKAKIIFENEGEEHLTTMEEMYERYKHLGEFYDEEYNRWGIDVSNVPIYVKSFDPESKRVVKGKVN
VIWKYELGKDVTKYEIITNKGTKILTSPWHPFFVLTPDFKIVEKRADELKEGDILIGGMPDGEDYKFIFD
YWLAGFIAGDGCFDKYHSHVKGHEYIYDRLRIYDYRIETFEIINDYLEKTFGRKYSIQKDRNIYYIDIKA
RNITSHYLKLLEGIDNGIPPQILKEGKNAVLSFIAGLFDAEGHVSNKPGIELGMVNKRLIEDVTHYLNAL
GIKARIREKLRKDGIDYVLHVEEYSSLLRFYELIGKNLQNEEKREKLEKVLSNHKGGNFGLPLNFNAFKE
WASEYGVEFKTNGSQTIAIINDERISLGQWHTRNRVSKAVLVKMLRKLYEATKDEEVKRMLHLIEGLEVV
RHITTTNEPRTFYDLTVENYQNYLAGENGMIFVHNT |
| Block A |
CIDGKAKIIFENE 13 |
| Block B |
GTKILTSPWHPFFV 103 |
| Block C |
LAGFIAGDG 150 |
| Block D |
DNGIPPQI 231 |
| Block E |
FIAGLFDAEG 251 |
| Block H |
MVNKRLIEDVTHYLNALGI 281 |
| Block F |
TFYDLTV**ENYQNYL 443 |
| Block G |
GMIFVHNT 455 |
| Initially Contributed by |
Frank Robb |
| Contributor's Address |
Center of Marine Biotechnology
University of Maryland
701 E. Pratt St
Baltimore, MD 21202, USA |
| Contributor's Phone No. |
1-410 234 8803 |
| Contributor's FAX No. |
1-410 234 8896 |
| Contributor's Email address |
robb@umbi.umd.edu |
| Independently Found By |
|
| Comments |
The Mja RNR inteins are in an anaerobic ribonucleoside-triphosphate reductase extein while the RIR1 inteins are in the alpha subunit of a ribonucleoside-diphosphate reductase extein. Endonuclease information from Y. Ishino. |
| Date Submitted |
|
| References |
Riera 1997
Yamazaki 1998
Maeder 1999
Komori 1999
Komori 1999B
Otomo 1999
Otomo 1999B
Ichiyanagi 2000
Iwai 2001 |
