| Intein Name |
Nsp-PCC7120 RIR1 |
| Prototype Allele |
Nsp-PCC7120 RIR1 |
| Extein Name |
Ribonucleoside Triphosphate Reductase, Class II |
| Intein Class |
Experimental |
| Organism Name |
Nostoc species PCC7120, (Anabaena sp. PCC7120) |
| Organism Description |
Cyanobacterium, Nitrogen-fixing, taxon:103690 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
R275 |
| Insert Site Comments |
RIR1-d |
| Intein Size (aa) |
407 |
| Intein N-terminal |
VTIVAGNIRR/C |
| Intein C-terminal |
HN/SAGMRQFISD |
| Accession No. |
NP_488075 in NCBI/protein |
| Intein aa Sequence |
>gi|17231527|ref|NP_488075.1|[17231527] Nsp-PCC7120 RIR1 intein (including -1 and +1 extein residues)
RCLPEDALVHTAKGLVPIRDVQVGDLVQTPLGFRRVVDKFDQGFQDVYEI
ETNATYPRATLNHRQAVLEDAKGGIVWKHIASLEAGDRLLHNKQVLPGTV
THLPADFTESRPSHSRTAKSFVVPELTAEVAWLIGFTHGDGYVALGRNKY
DKPYGRVEWSMNSLDAEVTSRIQAKIDAALALFGLSAVHSITKGENTAKS
ICSSIRLAEYFHRHIKQPNIPLTVPSFILQGSVDIRAAYLAGLMDSDGAV
NNRPPHLITSVYRSFIRQVSVVLSSLGIAGRLTTTYPQNSNWQVKYNLTI
PALKERYNALISPHSAKGELRQGLKMYGFTVPGAVMRETYTYSEMREMGF
QGSRTVDANYERYVAEADISLDIPVTVKGLGSYDHVQTYDIEVDEAHCFY
CDGYLTHNS |
| Block A |
CLPEDALVHTAKG 13 |
| Block B |
ATYPRATLNHRQAV 66 |
| Block C |
LIGFTHGDG 140 |
| Block D |
QPNIPLTV 223 |
| Block E |
YLAGLMDSDG 247 |
| Block H |
SVYRSFIRQVSVVLSSLGI 277 |
| Block F |
QTYDIEV**DEAHCFY 399 |
| Block G |
DGYLTHNS 408 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
|
| Contributor's Phone No. |
|
| Contributor's FAX No. |
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| Contributor's Email address |
|
| Independently Found By |
Florence K. Gleason
Department of Plant Biology
220 BioScience Center
University of Minnesota
St. Paul, MN 55108 USA
1-612-625-4275, FAX: 1-612-625-1738 florence@cbs.umn.edu |
| Comments |
Class II B12 dependent Ribonucleoside reductase
Extein matches to ribonucleotide reductases genes, especially from phage.
Ribonucleoside Triphosphate Reductase- B12 Dependent; experimental confirmation of the NspRIR intein (F.G.). |
| Date Submitted |
07/07/2002 |
| References |
Kaneko 2001
Gleason 2002 |
