| Intein Name |
Min-1442 DnaB |
| Prototype Allele |
Mle-TN DnaB |
| Extein Name |
DnaB helicase |
| Intein Class |
Theoretical |
| Organism Name |
Mycobacterium intracellulare |
| Organism Description |
strain 1442, taxon:1767 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K233 |
| Insert Site Comments |
dnaB-b, ATP/GTP-binding site motif A (P-loop) |
| Intein Size (aa) |
335 |
| Intein N-terminal |
IVAARPGVGK/A |
| Intein C-terminal |
HN/STLGLDFLRS |
| Accession No. |
AAG31144.1 in NCBI/protein |
| Intein aa Sequence |
>gi|11127927|gb|AAG31144.1|AF307984.1 Min-1442 DnaB intein (including -1 and +1 extein residues)
KALALDTPLPTPTGWTTTGDVAVGDELLGADGKPTRVVAATEVLLGRPCYEVEFSDGTVI
VADAAHQWLTETRASRKSAQAAAAGYNRYKNQRTFAAVRTTAEIAGTLRCPTLDRRLNHS
VVNARALDLPDREFLVPPYTLGAWLGDGTSAAAQITAADPEIIMRIEAEMSSTVGTLQAR
LRTIGVLGNKHIPTEYLRGSETQRRELLAGLLDTDGTVTAGGAVQFSVTNQRLACDVAEL
IVSLGYRCQTSTKRVRGRNESSSIAYTLTFSTEKKMFALERKAIHKERRAMAGTTRSGSR
FIVDVRPVETVAVRCVEVDNGSHLYLASRAMVPTHNS |
| Block A |
ALALDTPLPTPTG 13 |
| Block B |
GTVIVADAAHQWLT 69 |
| Block C |
TLGAWLGDG 147 |
| Block D |
NKHIPTEY 195 |
| Block E |
LLAGLLDTDG 215 |
| Block H |
VTNQRLACDVAELIVSLGY 245 |
| Block F |
AVRCVEV*DNGSHLYL 325 |
| Block G |
AMVPTHNS 336 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
New England Biolabs, Inc. |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
perler@neb.com |
| Independently Found By |
|
| Comments |
Has an Ala instead of the conserved intein N-terminal Ser/Cys.
Partial Extein sequence in Gi:11127927.
Insertion site based on Min DnaB gene without intein (GI:12958157) AAK09264 |
| Date Submitted |
02/08/2001 |
| References |
Yamamoto 2001
Yamamoto 2004 |
