| Intein Name |
Tli Pol-2 |
| Prototype Allele |
Tli Pol-2 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Thermococcus litoralis |
| Organism Description |
Thermophile, taxon:2265 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
PI-TliI |
| Endo Motif |
DOD |
| Location in Extein |
D1081 |
| Insert Site Comments |
pol-c, Pol Motif C |
| Intein Size (aa) |
390 |
| Intein N-terminal |
KRGFKVLYAD/S |
| Intein C-terminal |
HN/TDGFYATIPG |
| Accession No. |
S42459 in NCBI/protein |
| Intein aa Sequence |
>gi|543522|pir||S42459 Tli Pol-2 intein (including -1 and +1 extein residues)
DSVSGESEIIIRQNGKIRFVKIKDLFSKVDYSIGEKEYCILEGVEALTLDDDGKLVWKPVPYVMRHRANK
RMFRIWLTNSWYIDVTEDHSLIGYLNTSKTKTAKKIGERLKEVKPFELGKAVKSLICPNAPLKDENTKTS
EIAVKFWELVGLIVGDGNWGGDSRWAEYYLGLSTGKDAEEIKQKLLEPLKTYGVISNYYPKNEKGDFNIL
AKSLVKFMKRHFKDEKGRRKIPEFMYELPVTYIEAFLRGLFSADGTVTIRKGVPEIRLTNIDADFLREVR
KLLWIVGISNSIFAETTPNRYNGVSTGTYSKHLRIKNKWRFAERIGFLIERKQKRLLEHLKSARVKRNTI
DFGFDLVHVKKVEEIPYEGYVYDIEVEETHRFFANNILVHNT |
| Block A |
SVSGESEIIIRQN 13 |
| Block B |
SWYIDVTEDHSLIG 92 |
| Block C |
LVGLIVGDG 156 |
| Block D |
RRKIPEFM 234 |
| Block E |
FLRGLFSADG 254 |
| Block H |
NIDADFLREVRKLLWIVGI 287 |
| Block F |
YVYDIEV**EETHRFF 382 |
| Block G |
NNILVHNT 391 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
New England Biolabs |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
perler@neb.com |
| Independently Found By |
|
| Comments |
Using the convention of naming endos in order of discovery, Tli Pol-2 encodes PI-TliI (Perler). |
| Date Submitted |
|
| References |
Perler 1992
Hodges 1992
Saves 2000C |
|
