| Intein Name |
Hsp-NRC1 Pol-II |
| Prototype Allele |
Pho Pol-II |
| Extein Name |
DNA polymerase II, DP2 subunit |
| Intein Class |
Theoretical |
| Organism Name |
Halobacterium salinarum NRC-1 |
| Organism Description |
Halophile, taxon:64091 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
None |
| Location in Extein |
N925 |
| Insert Site Comments |
pol_II-a |
| Intein Size (aa) |
195 |
| Intein N-terminal |
PYFHAAKRRN/C |
| Intein C-terminal |
GQ/CDGDEDCVML |
| Accession No. |
NP_280963 in NCBI/protein, VNG2338G, polA2 |
| Intein aa Sequence |
>gi|10581749|gb|AAG20443.1|NP_280963|[10581749] Hsp-NRC1 Pol-II intein (including -1 and +1 extein residues)
NCFHPETNVWFRDESGEWHHDPIETLVEARLDPDTADEDDFGALVQALDGDVFVPSVTED
GEETLQRVEAVSKHPAPDHLLAVETKRGRELTVTPDHSMRRWTGDGIERVDARELTAGDA
LPAPTQVPGDGETATSELRSESLDGTHPQRRFGDGGSVRTDEVVSVEPVRSSVDHTYSLT
VAETNTLVANGLFTGQC |
| Block A |
CFHPETNVWFRDE 13 |
| Block B |
GRELTVTPDHSMRR 100 |
| Block C |
None |
| Block D |
None |
| Block E |
None |
| Block H |
None |
| Block F |
HTYSLTV***AETNTL 186 |
| Block G |
NGLFTGQC 196 |
| Initially Contributed by |
Isaac Cann |
| Contributor's Address |
New England Biolabs |
| Contributor's Phone No. |
1-978-927-5054 |
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
perler@neb.com |
| Independently Found By |
|
| Comments |
Formerly Halobacterium sp. NRC-1. The amino acid sequence of this intein shows less than 20% identity to its alleles in Pho and Pab. It has a C-terminal splice junction of GQ/C. DNA polymerase type II large chain |
| Date Submitted |
10/18/2000 |
| References |
Ng 2000 |
