New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
BsuP-M1918 RIR1 Intein
Intein Name BsuP-M1918 RIR1
Prototype Allele CIV RIR1
Extein Name Ribonucleoside- diphosphate reductase, alpha subunit
Intein Class Theoretical
Organism Name B.subtilis M1918 (prophage)
Organism Description Prophage in B.subtilis M1918. taxon:157928
Domain of Life Eubacteria
Endonuclease Activity
Endo Motif DOD
Location in Extein L30?
Insert Site Comments RIR1-i
Intein Size (aa) 385
Intein N-terminal HISKVKFSNL/C
Intein C-terminal GN/CSEVLQSSQV
Accession No. CAC51100.1 in NCBI/protein, bnrdE
Intein aa Sequence >gi|15211863|emb|CAC51100.1|[15211863] BsuP-M1918 RIR1 intein (including -1 and +1 extein residues)
LCVTGETLLLTENGYEKAADLYKKQNNLKVVIDNRTKDFAVDSKGTTIVDAIPMQLTKKD
AEIFKVKTKQGYEIRATEWHKFYVKRDGEIQKLQLNQLKTGDKLLVQSAEGAYGKIHEPD
LAYIMGIIAGDGTITEKTAKIYLYDNKKVLEQKVTDAVHRIIHKHKVDRAYKHNTSLLPT
FNMANPEKQDLLYMNSTVLFDILKKFGMNKERKTRVPEFIFQANKETQAAYLSGLFQTDG
CVNANHKAKALTIELTSIHYESLQDVQKLLLNMGVYTTIYSNNKRSQELLPDGKGGSKLY
NVKPTHKISIQDRNSRELFMSIVELKDYDVYKFNLLTETLQPKSRKPKHDFTAEIISIEE
DGVEDVYDTTQEDYHSLIFNGIVTGNC
Block A CVTGETLLLTENG 13
Block B GYEIRATEWHKFYV 83
Block C IMGIIAGDG 131
Block D KTRVPEFI 219
Block E YLSGLFQTDG 239
Block H SIHYESLQDVQKLLLNMGV 274
Block F DVYDTTQ**EDYHSLI 377
Block G NGIVTGNC 386
Initially Contributed by Fran Perler
Contributor's Address
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address
Independently Found By
Comments This intein has 2 aa differences compared to the Spb RIR1 intein from B. sutilis strain 168 and Spbeta c2 prophage.
Partial extein sequence.
Date Submitted 10/11/2001
References Lazarevic 2001

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home